Command line: /Users/Mike_Lee/happy_bin/SPAdes-3.11.1-Darwin/bin/spades.py -1 /Users/Mike_Lee/working_genomics_de_novo_temp/BCep_R1_QCd_err_cor.fastq.gz -2 /Users/Mike_Lee/working_genomics_de_novo_temp/BCep_R2_QCd_err_cor.fastq.gz -t 4 -o /Users/Mike_Lee/working_genomics_de_novo_temp/spades_kmers_set_careful_assembly -k 21,33,55,77 --careful --only-assembler System information: SPAdes version: 3.11.1 Python version: 2.7.10 OS: Darwin-17.3.0-x86_64-i386-64bit Output dir: /Users/Mike_Lee/working_genomics_de_novo_temp/spades_kmers_set_careful_assembly Mode: ONLY assembling (without read error correction) Debug mode is turned OFF Dataset parameters: Multi-cell mode (you should set '--sc' flag if input data was obtained with MDA (single-cell) technology or --meta flag if processing metagenomic dataset) Reads: Library number: 1, library type: paired-end orientation: fr left reads: ['/Users/Mike_Lee/working_genomics_de_novo_temp/BCep_R1_QCd_err_cor.fastq.gz'] right reads: ['/Users/Mike_Lee/working_genomics_de_novo_temp/BCep_R2_QCd_err_cor.fastq.gz'] interlaced reads: not specified single reads: not specified Assembly parameters: k: [21, 33, 55, 77] Repeat resolution is enabled Mismatch careful mode is turned ON MismatchCorrector will be used Coverage cutoff is turned OFF Other parameters: Dir for temp files: /Users/Mike_Lee/working_genomics_de_novo_temp/spades_kmers_set_careful_assembly/tmp Threads: 4 Memory limit (in Gb): 250