MEIC23-Escherichia-coli-strain-MOD1-EC898-origamin - 4 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

Escherichia-coli-strain-MOD1-EC898-chromosome-circular

Region	Type	From	To
Region 1.1	NRPS,T1PKS	433,020	488,484
Region 1.2	siderophore	1,513,763	1,528,177
Region 1.3	NRPS	3,704,736	3,748,617
Region 1.4	thiopeptide	4,082,404	4,108,696

Region	Type	From	To	Similarity
Region 1.1	NRPS,T1PKS	433,020	488,484
Region 1.2	siderophore	1,513,763	1,528,177
Region 1.3	NRPS	3,704,736	3,748,617
Region 1.4	thiopeptide	4,082,404	4,108,696

No secondary metabolite regions were found in these records:: Escherichia-coli-strain-MOD1-EC898-plasmid-circular; Escherichia-coli-strain-MOD1-EC898-scaffold_1; Escherichia-coli-strain-MOD1-EC898-scaffold_2; Escherichia-coli-strain-MOD1-EC898-scaffold_3; Escherichia-coli-strain-MOD1-EC898-scaffold_4; Escherichia-coli-strain-MOD1-EC898-scaffold_5

Compact view

Escherichia-coli-strain-MOD1-EC898-chromosome-circular - Region 1 - NRPS,T1PKS

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Location: 433,020 - 488,484 nt. (total: 55,465 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 433019 - 488484:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys) + (Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 433019 - 478783:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys) + (Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 438992 - 488484:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys) + (Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1_483

Search NORINE for peptide: strict or relaxed

AMP-binding (573..966): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSMIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

1_484

PKS_AT (552..842): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.0
Methoxymalonyl-CoA: 49.3
inactive: 45.5
Methylmalonyl-CoA: 42.8
Benzoyl-CoA: 42.7
Propionyl-CoA: 33.6
Isobutyryl-CoA: 30.8
fatty_acid: 25.6
Acetyl-CoA: 25.3
2-Methylbutyryl-CoA: 24.6
3-Methylbutyryl-CoA: 19.5
CHC-CoA: 16.3
trans-1,2-CPDA: 11.2
Ethylmalonyl-CoA: 6.5

1_487

Search NORINE for peptide: strict or relaxed

AMP-binding (20..422): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLCK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

Escherichia-coli-strain-MOD1-EC898-chromosome-circular - Region 2 - siderophore

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Location: 1,513,763 - 1,528,177 nt. (total: 14,415 nt) Show pHMM detection rules used

siderophore: IucA_IucC

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Escherichia-coli-strain-MOD1-EC898-chromosome-circular - Region 3 - NRPS

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Location: 3,704,736 - 3,748,617 nt. (total: 43,882 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 3704735 - 3748617:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1_3511

Search NORINE for peptide: strict or relaxed

AMP-binding (461..863): ser

NRPSPredictor2: ser

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

1_3519

Search NORINE for peptide: strict or relaxed

AMP-binding (36..437): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: AMPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

Escherichia-coli-strain-MOD1-EC898-chromosome-circular - Region 4 - thiopeptide

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Location: 4,082,404 - 4,108,696 nt. (total: 26,293 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03882) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

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core biosynthetic genes

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Thiopeptides

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.