ENGE22-1_SAMN26001779_MAG_00000007 - 15 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	LAP	183,272	208,186

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_22

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	T3PKS	54,286	87,627

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26

Region	Type	From	To	Most similar known cluster		Similarity
Region 26.1	NRPS,T1PKS,NRPS-like	1	77,115

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_31

Region	Type	From	To	Most similar known cluster		Similarity
Region 31.1	lanthipeptide-class-i,lanthipeptide-class-ii	1	20,562

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_34

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	lanthipeptide-class-iv	1	16,132

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_36

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	ladderane	5,501	48,010

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_42

Region	Type	From	To	Most similar known cluster		Similarity
Region 42.1	terpene	32,275	54,221

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_47

Region	Type	From	To	Most similar known cluster		Similarity
Region 47.1	terpene	31,054	51,992

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_55

Region	Type	From	To	Most similar known cluster		Similarity
Region 55.1	NRPS	1	33,028

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_61

Region	Type	From	To	Most similar known cluster		Similarity
Region 61.1	lassopeptide	21,762	38,403

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_76

Region	Type	From	To	Most similar known cluster		Similarity
Region 76.1	T3PKS	3,595	31,535

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_95

Region	Type	From	To	Most similar known cluster		Similarity
Region 95.1	ranthipeptide	1	25,756

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_103

Region	Type	From	To	Most similar known cluster		Similarity
Region 103.1	lanthipeptide-class-ii	1	16,994

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_122

Region	Type	From	To	Most similar known cluster		Similarity
Region 122.1	lanthipeptide-class-i	1	17,455

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_137

Region	Type	From	To	Most similar known cluster		Similarity
Region 137.1	NRPS-like	1	13,974

Region	Type	From	To	Similarity
Region 2.1	LAP	183,272	208,186
Region 22.1	T3PKS	54,286	87,627
Region 26.1	NRPS,T1PKS,NRPS-like	1	77,115
Region 31.1	lanthipeptide-class-i,lanthipeptide-class-ii	1	20,562
Region 34.1	lanthipeptide-class-iv	1	16,132
Region 36.1	ladderane	5,501	48,010
Region 42.1	terpene	32,275	54,221
Region 47.1	terpene	31,054	51,992
Region 55.1	NRPS	1	33,028
Region 61.1	lassopeptide	21,762	38,403
Region 76.1	T3PKS	3,595	31,535
Region 95.1	ranthipeptide	1	25,756
Region 103.1	lanthipeptide-class-ii	1	16,994
Region 122.1	lanthipeptide-class-i	1	17,455
Region 137.1	NRPS-like	1	13,974

No secondary metabolite regions were found in these records:: ENGE22-1_SAMN26001779_MAG_00000007-scaffold_1; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_3; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_4; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_5; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_6; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_7; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_8; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_9; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_10; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_11; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_12; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_13; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_14; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_15; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_16; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_17; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_18; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_19; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_20; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_21; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_23; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_24; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_25; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_27; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_28; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_29; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_30; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_32; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_33; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_35; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_37; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_38; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_39; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_40; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_41; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_43; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_44; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_45; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_46; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_48; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_49; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_50; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_51; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_52; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_53; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_54; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_56; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_57; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_58; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_59; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_60; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_62; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_63; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_64; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_65; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_66; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_67; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_68; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_69; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_70; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_71; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_72; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_73; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_74; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_75; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_77; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_78; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_79; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_80; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_81; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_82; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_83; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_84; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_85; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_86; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_87; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_88; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_89; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_90; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_91; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_92; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_93; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_94; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_96; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_97; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_98; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_99; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_100; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_101; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_102; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_104; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_105; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_106; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_107; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_108; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_109; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_110; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_111; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_112; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_113; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_114; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_115; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_116; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_117; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_118; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_119; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_120; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_121; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_123; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_124; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_125; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_126; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_127; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_128; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_129; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_130; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_131; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_132; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_133; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_134; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_135; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_136; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_138; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_139; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_140; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_141; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_142; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_143; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_144; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_145; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_146; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_147; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_148; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_149; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_150; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_151; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_152; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_153; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_154; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_155; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_156; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_157; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_158; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_159; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_160; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_161; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_162; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_163; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_164; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_165; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_166; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_167; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_168; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_169; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_170; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_171; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_172; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_173; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_174; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_175; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_176; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_177; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_178; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_179; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_180; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_181; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_182; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_183; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_184; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_185; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_186; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_187; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_188; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_189; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_190; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_191; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_192; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_193; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_194; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_195; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_196; ENGE22-1_SAMN26001779_MAG_00000007-scaffold_197

Compact view

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_2 - Region 1 - LAP

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Location: 183,272 - 208,186 nt. (total: 24,915 nt) Show pHMM detection rules used

Region on contig edge.

LAP: ((((goadsporin_like or PF00881 or TIGR03605) and (YcaO or TIGR03882 or TIGR03604)) or TIGR03603 or TIGR03602) and not cyanobactin_synth)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_22 - Region 1 - T3PKS

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Location: 54,286 - 87,627 nt. (total: 33,342 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26 - Region 1 - NRPS,NRPS-like,T1PKS

Download region GenBank file

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Location: 1 - 77,115 nt. (total: 77,115 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 77115:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - gln) + (ohmal) + (X) + (Me-ohmal) + (D-gln) + (D-thr) + (leu - asn) + (pro) + (tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 42505 - 77115:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-thr) + (leu - asn) + (pro) + (tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_13

Search NORINE for peptide: strict or relaxed

CAL_domain (49..484): pk

minowa: NH2

Prediction, score:

NH2: 135.6
fatty_acid: 17.1
shikimic_acid: 4.2
AHBA: 2.6
Acetyl-CoA: -1.7

PKS_AT (1223..1516): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 135.2
Methoxymalonyl-CoA: 79.3
Methylmalonyl-CoA: 77.8
inactive: 59.8
Ethylmalonyl-CoA: 54.6
Propionyl-CoA: 51.5
2-Methylbutyryl-CoA: 41.6
trans-1,2-CPDA: 36.5
Acetyl-CoA: 33.0
CHC-CoA: 31.9
Isobutyryl-CoA: 28.1
fatty_acid: 22.5
Benzoyl-CoA: 21.7
3-Methylbutyryl-CoA: 13.2

AMP-binding (2990..3389): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLvDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_14

PKS_AT (536..825): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 114.5
Methoxymalonyl-CoA: 65.4
Methylmalonyl-CoA: 61.0
inactive: 57.2
Propionyl-CoA: 44.9
Ethylmalonyl-CoA: 39.9
2-Methylbutyryl-CoA: 34.6
Acetyl-CoA: 26.5
CHC-CoA: 21.8
Isobutyryl-CoA: 21.3
fatty_acid: 19.5
Benzoyl-CoA: 14.1
3-Methylbutyryl-CoA: 13.0
trans-1,2-CPDA: 12.2

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_15

Search NORINE for peptide: strict or relaxed

AMP-binding (528..961): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DlWSVGfVwK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_16

PKS_AT (532..821): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 114.5
Methoxymalonyl-CoA: 65.4
Methylmalonyl-CoA: 61.0
inactive: 57.2
Propionyl-CoA: 44.9
Ethylmalonyl-CoA: 39.9
2-Methylbutyryl-CoA: 34.6
Acetyl-CoA: 26.5
CHC-CoA: 21.8
Isobutyryl-CoA: 21.3
fatty_acid: 19.5
Benzoyl-CoA: 14.1
3-Methylbutyryl-CoA: 13.0
trans-1,2-CPDA: 12.2

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_17

Search NORINE for peptide: strict or relaxed

AMP-binding (537..946): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGlVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_18

Search NORINE for peptide: strict or relaxed

AMP-binding (949..1349): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_19

Search NORINE for peptide: strict or relaxed

AMP-binding (467..858): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFhGfVcK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 70% (weak)

AMP-binding (1512..1911): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_20

Search NORINE for peptide: strict or relaxed

AMP-binding (545..945): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFyAHLAK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 90% (moderate)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_26_22

Search NORINE for peptide: strict or relaxed

AMP-binding (267..660): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_31 - Region 1 - lanthipeptide-class-i,lanthipeptide-class-ii

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Location: 1 - 20,562 nt. (total: 20,562 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_34 - Region 1 - lanthipeptide-class-iv

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Location: 1 - 16,132 nt. (total: 16,132 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_36 - Region 1 - ladderane

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Location: 5,501 - 48,010 nt. (total: 42,510 nt) Show pHMM detection rules used

ladderane: ladderane

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ENGE22-1_SAMN26001779_MAG_00000007-scaffold_42 - Region 1 - terpene

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Location: 32,275 - 54,221 nt. (total: 21,947 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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ENGE22-1_SAMN26001779_MAG_00000007-scaffold_47 - Region 1 - terpene

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Location: 31,054 - 51,992 nt. (total: 20,939 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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ENGE22-1_SAMN26001779_MAG_00000007-scaffold_55 - Region 1 - NRPS

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Location: 1 - 33,028 nt. (total: 33,028 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 33028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_55_2

Search NORINE for peptide: strict or relaxed

AMP-binding (510..908): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLiAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_55_7

Search NORINE for peptide: strict or relaxed

AMP-binding (453..845): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_55_9

Search NORINE for peptide: strict or relaxed

AMP-binding (4..424): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: a-FYLavvCK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_61 - Region 1 - lassopeptide

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Location: 21,762 - 38,403 nt. (total: 16,642 nt) Show pHMM detection rules used

Region on contig edge.

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

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Lasso peptides

Lasso peptide predictions

Lassopeptide(s) for ENGE22-1_SAMN26001779_MAG_00000007-scaffold_61_26 - Putative Class II

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_61_26 leader / core peptide / cleaved tail: MKQYSAPELFELGHAADLTK - AFVGGTIADNDCVYRDGWA

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_61_26 - Class II: Cleavage pHMM score: 7.90; RODEO score: 19; Monoisotopic mass: 2010.9 Da; Molecular weight: 2012.2 Da; Number of bridges: 0

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_76 - Region 1 - T3PKS

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Location: 3,595 - 31,535 nt. (total: 27,941 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_95 - Region 1 - ranthipeptide

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Location: 1 - 25,756 nt. (total: 25,756 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or YcaO or TIGR03882)))

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ENGE22-1_SAMN26001779_MAG_00000007-scaffold_103 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 16,994 nt. (total: 16,994 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptide(s) for ENGE22-1_SAMN26001779_MAG_00000007-scaffold_103_4 - Putative Class II

allorf_03839_04030 leader / core peptide: LERSVSIQPCHHTGRDDQVQLA - VGEVIQAVDhbCRLHDWPDhaAAVERRVDQPRNDhbGLGFEELQEVV
allorf_12103_12252 leader / core peptide: LLDRSAENSPNREALPLA - QPLRCRCDhaDhaKQRDhaFLDNWKGDhbVRQAPVPDhbAE

Lanthipeptide(s) for ENGE22-1_SAMN26001779_MAG_00000007-scaffold_103_6 - Putative Class II

allorf_03839_04030 leader / core peptide: LERSVSIQPCHHTGRDDQVQLA - VGEVIQAVDhbCRLHDWPDhaAAVERRVDQPRNDhbGLGFEELQEVV
allorf_12103_12252 leader / core peptide: LLDRSAENSPNREALPLA - QPLRCRCDhaDhaKQRDhaFLDNWKGDhbVRQAPVPDhbAE

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_03839_04030 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 4548.3 Da

Molecular weight: 4551.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4569.1 Da N = 1; 4587.1 Da N = 2

allorf_12103_12252 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 3467.8 Da

Molecular weight: 3469.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3488.0 Da N = 1; 3506.0 Da N = 2; 3524.0 Da N = 3

allorf_03839_04030 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 4548.3 Da

Molecular weight: 4551.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4569.1 Da N = 1; 4587.1 Da N = 2

allorf_12103_12252 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 3467.8 Da

Molecular weight: 3469.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3488.0 Da N = 1; 3506.0 Da N = 2; 3524.0 Da N = 3

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_122 - Region 1 - lanthipeptide-class-i

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Location: 1 - 17,455 nt. (total: 17,455 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_137 - Region 1 - NRPS-like

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Location: 1 - 13,974 nt. (total: 13,974 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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Module view

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 13974:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ENGE22-1_SAMN26001779_MAG_00000007-scaffold_137_4

Search NORINE for peptide: strict or relaxed

AMP-binding (529..965): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: eqvvaGGPvK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 40% (weak)