CARD22-1_SAMN24292799_MAG_00000014 - 18 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

CARD22-1_SAMN24292799_MAG_00000014-scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	phosphonate	254,997	278,509

CARD22-1_SAMN24292799_MAG_00000014-scaffold_4

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	hglE-KS	186,357	214,173

CARD22-1_SAMN24292799_MAG_00000014-scaffold_7

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS,T1PKS	7,432	73,426

CARD22-1_SAMN24292799_MAG_00000014-scaffold_9

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	NRPS	177	43,401
Region 9.2	thiopeptide	70,079	97,795

CARD22-1_SAMN24292799_MAG_00000014-scaffold_15

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	T1PKS	35,213	86,978

CARD22-1_SAMN24292799_MAG_00000014-scaffold_17

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	betalactone	20,155	43,909

CARD22-1_SAMN24292799_MAG_00000014-scaffold_30

Region	Type	From	To	Most similar known cluster		Similarity
Region 30.1	acyl_amino_acids	1	39,618

CARD22-1_SAMN24292799_MAG_00000014-scaffold_33

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	NRPS	1	45,091

CARD22-1_SAMN24292799_MAG_00000014-scaffold_38

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	NRPS,ladderane	1	39,813

CARD22-1_SAMN24292799_MAG_00000014-scaffold_51

Region	Type	From	To	Most similar known cluster		Similarity
Region 51.1	NRPS-like	18,135	40,773

CARD22-1_SAMN24292799_MAG_00000014-scaffold_60

Region	Type	From	To	Most similar known cluster		Similarity
Region 60.1	T1PKS	7,388	36,538

CARD22-1_SAMN24292799_MAG_00000014-scaffold_63

Region	Type	From	To	Most similar known cluster		Similarity
Region 63.1	RRE-containing,RiPP-like	18,408	34,993

CARD22-1_SAMN24292799_MAG_00000014-scaffold_69

Region	Type	From	To	Most similar known cluster		Similarity
Region 69.1	RRE-containing	1	18,965

CARD22-1_SAMN24292799_MAG_00000014-scaffold_70

Region	Type	From	To	Most similar known cluster		Similarity
Region 70.1	acyl_amino_acids,hserlactone	1	26,931

CARD22-1_SAMN24292799_MAG_00000014-scaffold_78

Region	Type	From	To	Most similar known cluster		Similarity
Region 78.1	NRPS,betalactone,transAT-PKS	1	20,852

CARD22-1_SAMN24292799_MAG_00000014-scaffold_99

Region	Type	From	To	Most similar known cluster		Similarity
Region 99.1	RRE-containing	1	9,067

CARD22-1_SAMN24292799_MAG_00000014-scaffold_100

Region	Type	From	To	Most similar known cluster		Similarity
Region 100.1	NRPS-like	1	8,629

Region	Type	From	To	Similarity
Region 2.1	phosphonate	254,997	278,509
Region 4.1	hglE-KS	186,357	214,173
Region 7.1	NRPS,T1PKS	7,432	73,426
Region 9.1	NRPS	177	43,401
Region 9.2	thiopeptide	70,079	97,795
Region 15.1	T1PKS	35,213	86,978
Region 17.1	betalactone	20,155	43,909
Region 30.1	acyl_amino_acids	1	39,618
Region 33.1	NRPS	1	45,091
Region 38.1	NRPS,ladderane	1	39,813
Region 51.1	NRPS-like	18,135	40,773
Region 60.1	T1PKS	7,388	36,538
Region 63.1	RRE-containing,RiPP-like	18,408	34,993
Region 69.1	RRE-containing	1	18,965
Region 70.1	acyl_amino_acids,hserlactone	1	26,931
Region 78.1	NRPS,betalactone,transAT-PKS	1	20,852
Region 99.1	RRE-containing	1	9,067
Region 100.1	NRPS-like	1	8,629

No secondary metabolite regions were found in these records:: CARD22-1_SAMN24292799_MAG_00000014-scaffold_1; CARD22-1_SAMN24292799_MAG_00000014-scaffold_3; CARD22-1_SAMN24292799_MAG_00000014-scaffold_5; CARD22-1_SAMN24292799_MAG_00000014-scaffold_6; CARD22-1_SAMN24292799_MAG_00000014-scaffold_8; CARD22-1_SAMN24292799_MAG_00000014-scaffold_10; CARD22-1_SAMN24292799_MAG_00000014-scaffold_11; CARD22-1_SAMN24292799_MAG_00000014-scaffold_12; CARD22-1_SAMN24292799_MAG_00000014-scaffold_13; CARD22-1_SAMN24292799_MAG_00000014-scaffold_14; CARD22-1_SAMN24292799_MAG_00000014-scaffold_16; CARD22-1_SAMN24292799_MAG_00000014-scaffold_18; CARD22-1_SAMN24292799_MAG_00000014-scaffold_19; CARD22-1_SAMN24292799_MAG_00000014-scaffold_20; CARD22-1_SAMN24292799_MAG_00000014-scaffold_21; CARD22-1_SAMN24292799_MAG_00000014-scaffold_22; CARD22-1_SAMN24292799_MAG_00000014-scaffold_23; CARD22-1_SAMN24292799_MAG_00000014-scaffold_24; CARD22-1_SAMN24292799_MAG_00000014-scaffold_25; CARD22-1_SAMN24292799_MAG_00000014-scaffold_26; CARD22-1_SAMN24292799_MAG_00000014-scaffold_27; CARD22-1_SAMN24292799_MAG_00000014-scaffold_28; CARD22-1_SAMN24292799_MAG_00000014-scaffold_29; CARD22-1_SAMN24292799_MAG_00000014-scaffold_31; CARD22-1_SAMN24292799_MAG_00000014-scaffold_32; CARD22-1_SAMN24292799_MAG_00000014-scaffold_34; CARD22-1_SAMN24292799_MAG_00000014-scaffold_35; CARD22-1_SAMN24292799_MAG_00000014-scaffold_36; CARD22-1_SAMN24292799_MAG_00000014-scaffold_37; CARD22-1_SAMN24292799_MAG_00000014-scaffold_39; CARD22-1_SAMN24292799_MAG_00000014-scaffold_40; CARD22-1_SAMN24292799_MAG_00000014-scaffold_41; CARD22-1_SAMN24292799_MAG_00000014-scaffold_42; CARD22-1_SAMN24292799_MAG_00000014-scaffold_43; CARD22-1_SAMN24292799_MAG_00000014-scaffold_44; CARD22-1_SAMN24292799_MAG_00000014-scaffold_45; CARD22-1_SAMN24292799_MAG_00000014-scaffold_46; CARD22-1_SAMN24292799_MAG_00000014-scaffold_47; CARD22-1_SAMN24292799_MAG_00000014-scaffold_48; CARD22-1_SAMN24292799_MAG_00000014-scaffold_49; CARD22-1_SAMN24292799_MAG_00000014-scaffold_50; CARD22-1_SAMN24292799_MAG_00000014-scaffold_52; CARD22-1_SAMN24292799_MAG_00000014-scaffold_53; CARD22-1_SAMN24292799_MAG_00000014-scaffold_54; CARD22-1_SAMN24292799_MAG_00000014-scaffold_55; CARD22-1_SAMN24292799_MAG_00000014-scaffold_56; CARD22-1_SAMN24292799_MAG_00000014-scaffold_57; CARD22-1_SAMN24292799_MAG_00000014-scaffold_58; CARD22-1_SAMN24292799_MAG_00000014-scaffold_59; CARD22-1_SAMN24292799_MAG_00000014-scaffold_61; CARD22-1_SAMN24292799_MAG_00000014-scaffold_62; CARD22-1_SAMN24292799_MAG_00000014-scaffold_64; CARD22-1_SAMN24292799_MAG_00000014-scaffold_65; CARD22-1_SAMN24292799_MAG_00000014-scaffold_66; CARD22-1_SAMN24292799_MAG_00000014-scaffold_67; CARD22-1_SAMN24292799_MAG_00000014-scaffold_68; CARD22-1_SAMN24292799_MAG_00000014-scaffold_71; CARD22-1_SAMN24292799_MAG_00000014-scaffold_72; CARD22-1_SAMN24292799_MAG_00000014-scaffold_73; CARD22-1_SAMN24292799_MAG_00000014-scaffold_74; CARD22-1_SAMN24292799_MAG_00000014-scaffold_75; CARD22-1_SAMN24292799_MAG_00000014-scaffold_76; CARD22-1_SAMN24292799_MAG_00000014-scaffold_77; CARD22-1_SAMN24292799_MAG_00000014-scaffold_79; CARD22-1_SAMN24292799_MAG_00000014-scaffold_80; CARD22-1_SAMN24292799_MAG_00000014-scaffold_81; CARD22-1_SAMN24292799_MAG_00000014-scaffold_82; CARD22-1_SAMN24292799_MAG_00000014-scaffold_83; CARD22-1_SAMN24292799_MAG_00000014-scaffold_84; CARD22-1_SAMN24292799_MAG_00000014-scaffold_85; CARD22-1_SAMN24292799_MAG_00000014-scaffold_86; CARD22-1_SAMN24292799_MAG_00000014-scaffold_87; CARD22-1_SAMN24292799_MAG_00000014-scaffold_88; CARD22-1_SAMN24292799_MAG_00000014-scaffold_89; CARD22-1_SAMN24292799_MAG_00000014-scaffold_90; CARD22-1_SAMN24292799_MAG_00000014-scaffold_91; CARD22-1_SAMN24292799_MAG_00000014-scaffold_92; CARD22-1_SAMN24292799_MAG_00000014-scaffold_93; CARD22-1_SAMN24292799_MAG_00000014-scaffold_94; CARD22-1_SAMN24292799_MAG_00000014-scaffold_95; CARD22-1_SAMN24292799_MAG_00000014-scaffold_96; CARD22-1_SAMN24292799_MAG_00000014-scaffold_97; CARD22-1_SAMN24292799_MAG_00000014-scaffold_98; CARD22-1_SAMN24292799_MAG_00000014-scaffold_101; CARD22-1_SAMN24292799_MAG_00000014-scaffold_102; CARD22-1_SAMN24292799_MAG_00000014-scaffold_103; CARD22-1_SAMN24292799_MAG_00000014-scaffold_104; CARD22-1_SAMN24292799_MAG_00000014-scaffold_105; CARD22-1_SAMN24292799_MAG_00000014-scaffold_106; CARD22-1_SAMN24292799_MAG_00000014-scaffold_107; CARD22-1_SAMN24292799_MAG_00000014-scaffold_108

Compact view

CARD22-1_SAMN24292799_MAG_00000014-scaffold_2 - Region 1 - phosphonate

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Location: 254,997 - 278,509 nt. (total: 23,513 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: phosphonates

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_4 - Region 1 - hglE-KS

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Location: 186,357 - 214,173 nt. (total: 27,817 nt) Show pHMM detection rules used

Region on contig edge.

hglE-KS: (hglE or hglD)

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 186356 - 214173:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_4_193

PKS_AT (576..878): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 52.5
Methoxymalonyl-CoA: 44.0
inactive: 29.1
Methylmalonyl-CoA: 26.4
Propionyl-CoA: 16.6
fatty_acid: 15.7
Isobutyryl-CoA: 8.6
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

CARD22-1_SAMN24292799_MAG_00000014-scaffold_7 - Region 1 - NRPS,T1PKS

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Location: 7,432 - 73,426 nt. (total: 65,995 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 7431 - 73426:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - cys) + (cys) + (Me-cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_7_26

Search NORINE for peptide: strict or relaxed

PKS_AT (637..933): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.0
inactive: 151.1
Methoxymalonyl-CoA: 58.5
Methylmalonyl-CoA: 50.1
Propionyl-CoA: 37.1
Benzoyl-CoA: 29.2
Isobutyryl-CoA: 25.6
Acetyl-CoA: 19.6
2-Methylbutyryl-CoA: 17.2
CHC-CoA: 12.5
Ethylmalonyl-CoA: 9.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (2356..2757): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLiWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_7_29

Search NORINE for peptide: strict or relaxed

AMP-binding (2048..2451): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_7_30

Search NORINE for peptide: strict or relaxed

AMP-binding (542..941): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNMSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_9 - Region 1 - NRPS

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Location: 177 - 43,401 nt. (total: 43,225 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 176 - 43401:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_9_15

Search NORINE for peptide: strict or relaxed

AMP-binding (495..889): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: phe, trp
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAyTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 90% (moderate)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_9 - Region 2 - thiopeptide

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Location: 70,079 - 97,795 nt. (total: 27,717 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03882) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

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Thiopeptides

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.

CARD22-1_SAMN24292799_MAG_00000014-scaffold_15 - Region 1 - T1PKS

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Location: 35,213 - 86,978 nt. (total: 51,766 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

CARD22-1_SAMN24292799_MAG_00000014-scaffold_17 - Region 1 - betalactone

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Location: 20,155 - 43,909 nt. (total: 23,755 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_30 - Region 1 - acyl_amino_acids

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Location: 1 - 39,618 nt. (total: 39,618 nt) Show pHMM detection rules used

Region on contig edge.

acyl_amino_acids: NasY

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_33 - Region 1 - NRPS

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Location: 1 - 45,091 nt. (total: 45,091 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 45091:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-cys - cys - Me-cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_33_15

Search NORINE for peptide: strict or relaxed

AMP-binding (574..972): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

AMP-binding (2042..2447): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

AMP-binding (3112..3512): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_38 - Region 1 - NRPS,ladderane

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Location: 1 - 39,813 nt. (total: 39,813 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
ladderane: ladderane

Legend:

core biosynthetic genes

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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Module view

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 39813:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_38_9

Search NORINE for peptide: strict or relaxed

AMP-binding (8..411): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DmAgIGAlcK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 60% (weak)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_38_11

Search NORINE for peptide: strict or relaxed

CAL_domain (163..479): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 62.2
NH2: 44.3
Acetyl-CoA: 8.9
shikimic_acid: 0.0
AHBA: 0.0

AMP-binding (1134..1556): X

NRPSPredictor2: hydrophobic-aromatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: eAgTLGAvhK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 60% (weak)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_38_12

Search NORINE for peptide: strict or relaxed

AMP-binding (20..428): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWyLafVlK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_51 - Region 1 - NRPS-like

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Location: 18,135 - 40,773 nt. (total: 22,639 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 18134 - 40773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_51_30

Search NORINE for peptide: strict or relaxed

AMP-binding (2..369): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_60 - Region 1 - T1PKS

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Location: 7,388 - 36,538 nt. (total: 29,151 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 7387 - 36538:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_60_27

PKS_AT (547..842): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 83.3
Methylmalonyl-CoA: 78.7
Malonyl-CoA: 74.6
Isobutyryl-CoA: 74.3
Ethylmalonyl-CoA: 59.5
Benzoyl-CoA: 47.4
Propionyl-CoA: 40.1
Acetyl-CoA: 35.5
2-Methylbutyryl-CoA: 29.9
trans-1,2-CPDA: 27.6
fatty_acid: 24.6
inactive: 20.8
3-Methylbutyryl-CoA: 20.4
CHC-CoA: 15.3

CARD22-1_SAMN24292799_MAG_00000014-scaffold_63 - Region 1 - RRE-containing,RiPP-like

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Location: 18,408 - 34,993 nt. (total: 16,586 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_69 - Region 1 - RRE-containing

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Location: 1 - 18,965 nt. (total: 18,965 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_70 - Region 1 - acyl_amino_acids,hserlactone

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Location: 1 - 26,931 nt. (total: 26,931 nt) Show pHMM detection rules used

Region on contig edge.

acyl_amino_acids: NasY
hserlactone: Autoind_synth

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_78 - Region 1 - NRPS,betalactone,transAT-PKS

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Location: 1 - 20,852 nt. (total: 20,852 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

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Predicted core structure(s)

For candidate cluster 1, location 0 - 20852:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys) + (cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_78_1

PKS_AT (206..502): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.5
inactive: 137.1
Methoxymalonyl-CoA: 65.1
Methylmalonyl-CoA: 54.2
Propionyl-CoA: 39.5
Isobutyryl-CoA: 27.9
Benzoyl-CoA: 26.8
Acetyl-CoA: 20.9
2-Methylbutyryl-CoA: 19.0
Ethylmalonyl-CoA: 18.0
CHC-CoA: 14.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

CARD22-1_SAMN24292799_MAG_00000014-scaffold_78_2

Search NORINE for peptide: strict or relaxed

AMP-binding (30..434): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLCK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_78_4

Search NORINE for peptide: strict or relaxed

AMP-binding (568..965): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNfSMIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_78_5

Search NORINE for peptide: strict or relaxed

AMP-binding (1719..2118): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

CARD22-1_SAMN24292799_MAG_00000014-scaffold_99 - Region 1 - RRE-containing

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Location: 1 - 9,067 nt. (total: 9,067 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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CARD22-1_SAMN24292799_MAG_00000014-scaffold_100 - Region 1 - NRPS-like

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Location: 1 - 8,629 nt. (total: 8,629 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS domains

NRPS/PKS modules

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 8629:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

CARD22-1_SAMN24292799_MAG_00000014-scaffold_100_5

Search NORINE for peptide: strict or relaxed

AMP-binding (0..246): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)